LIV players such as Tyrrell Hatton and Jon Rahm have previously been allowed compete at European tour events.
Players will be banned from competing on the DP World Tour next season if they choose to join next year's LIV Golf.
Some of the world's top players in previous seasons have been given conditional releases by the European tour allowing them to compete.
LIV players such as Tyrrell Hatton and Jon Rahm have previously been allowed compete at European tour events.
But the Tour's CEO Guy Kinnings now says players must "make a choice" between the two circuits.
In order to be eligible for Europe's Ryder Cup team, they must be members of the DP World Tour.

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