Opposition parties have said the mass exodus of senior health officials raises "serious questions."
The abrupt resignation of HSE chief executive Paul Reid raises serious questions for Health Minister Stephen Donnelly, according to Social Democrats Health Spokesperson Róisín Shortall and Sinn Féin's Housing spokesperson Eoin O'Broin.
Co Leader of the Social Democrats, Ms Shortall commented:“Mr Reid, in a statement, stated that one of the reasons for his resignation was that the “HSE was entering a new phase and the appointment of a new leader was now timely.
"However, Mr Reid has always previously expressed enthusiasm about “leading the transformation” in healthcare services so it is unclear why that enthusiasm has now waned, two years before this contract was due to expire.
“The timing of Mr Reid’s resignation, in the midst of a controversy about the reconfiguration of hospital services at Navan Hospital in which Mr Reid and Mr Donnelly appear to be at complete odds - is notable.
"It begs the questions, has Mr Reid opted to leave because political support for health service reforms are absent?" she questioned.
Meanwhile, Sinn Fein's Eoin O'Broin has said questions have to be asked about the Health Ministers relationship with senior personnel in the HSE.
"This is the latest in a line of senior losses that Stephen Donnelly has presided over.
"From Tony Holohan to Ronan Glynn, and there's talk about Robert Watt's future being in some kind of question," said O'Broin.
"There's clearly something going on and some problem in terms of the Ministers relationship with Senior officials.
"We'll have to wait to hear more as the story unfolds, " he added.

Over 1,700 Speeding Offences Detected in May Bank Holiday
Kildare Man Charged With Criminal Damage By Arson Following Fatal House Fire
Young Kildare Delegates Attend Leinster House For Dáil na nÓg
First-Time Buyers Will Be Able To Borrow Up To Five Times Their Income
Only 65% Of Under 35s Recognise Risks Of Driving Morning After Drinking, As Bank Holiday Approaches
Worst Prison Overcrowding In History Of State, According To Prison Officers Association
Over 12,000 Defects Remain On New National Children's Hospital project.
Minister Martin Heydon Speaks As Fuel Scheme Announced